A simple, specific, accurate, precise and reproducible zero crossing difference spectrophotometric method has been developed and validated for the simultaneous estimation of Aspirin (ASP) and Dipyridamole (DIP) in their combined dosage form. Measurements of absorbance were carried out at zero-crossing wavelengths 318.6 and 251.6 nm for aspirin and dipyridamole by zero-crossing difference spectrophotometric method. Beer’s law is obeyed in the concentration range of 1–17 μg/mL (r2=0.9996) for aspirin and 3–23 μg/mL (r2=0.9999) for dipyridamole by zero-crossing difference spectrophotometric method. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The suitability of this method for quantitative determination of Aspirin and Dipyridamole was proved by validation and recovery studies. The method was validated as per International Conference on Harmonization (ICH) guideline in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to capsule formulation and no interference from the sample mixture’s excipients was found.
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